Modification of essential carboxyl residues in chloroplast coupling factor 1
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چکیده
منابع مشابه
Light-dependent modification of spinach chloroplast coupling factor 1 by permanganate ion.
Treatment of spinach chloroplasts with 25 to 100 pM potassium permanganate in the presence of light, magnesium, and ADP gives rise to 35 to 60% inhibition of the subsequent phosphorylation and ATPase activities. For half-maximal inhibition, 5 to 10 PM ADP is necessary and ADP is not replaceable by other nucleotides. The light requirement can be met by preillumination as well as by an acid bath ...
متن کاملChemical cross-linking studies of chloroplast coupling factor 1.
Cross-linking reagents have been used to link covalently adjacent subunits of solubilized spinach chloroplast coupling factor 1, which is a latent ATPase. 1,5-Difluoro-2,4-dinitrobenzene, dimethyl-3,3'-dithiobispropionimidate, and dimethylsuberimidate are able to form bridges of 3 to 11 A between amino groups, and hydrogen peroxide and the o-phenanthroline-cupric ion complex catalyze the oxidat...
متن کاملModification of Sulfhydryl Croups in the y-Subunit of Chloroplast-Coupling Factor
A large proton leak not coupled to ATP synthesis (slip) occurs at alkaline pH through the chloroplast ATP synthase (Y. Evron, M. Avron [1990] Biochim Biophys Acta 1019: 115-120). The involvement of the ATP synthase y-subunit in the regulation of proton conductance was analyzed by measuring the effect of thiolalkylating agents on proton slip. Alkylation by N-ethylmaleimide of y-cysteine (Cys)-89...
متن کاملChloroplast coupling factor 1: A species-specific receptor for tentoxin.
Tentoxin, a cyclic tetrapeptide, induces chlorosis in certain plant species. It inactivated photophosphorylation and coupling factor 1 (CF(1)) ATPase in lettuce, a sensitive species. This effect was due to binding of tentoxin with CF(1) at a single site (affinity constant 1.3 to 20 x 10(7) M(-1)). Neither AMP nor adenyl-5'-yl imidodiphosphate appeared to bind to this site. In radish, an insensi...
متن کاملFragmentation of chloroplast coupling factor in dependence of bound nucleotides
Previous studies on the ability of CF, fragments to reconstitute photophosphorylation in CF,-depleted thylakoids have shown that the degree of reconstitution was correlated with the presence of subunit 6 in the fragment. This was taken as evidence that subunit 6 was necessary for plugging the active proton channel CF, [(1986) Eur. J. Biochem. 160,635*3]. We questioned whether or not 6 alone had...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1980
ISSN: 0014-5793
DOI: 10.1016/0014-5793(80)80618-1